PCRBIO HiFi Polymerase

PCR Biosystems
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High fidelity • Enhanced processivity • Versatile


PCRBIO HiFi Polymerase was derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity in PCR. Several proprietary point mutations allow for significantly improved performance when compared with its native form. Together with advanced buffer chemistry this enzyme brings robust performance to the world of high fidelity PCR. Enhanced DNA binding allows for improved processivity, increasing yield and shortening cycling times. The enhanced efficiency of PCRBIO HiFi Polymerase minimises PCR inhibition, from impure samples such as colony PCR and direct PCR.

PCRBIO HiFi Polymerase uses the latest developments in DNA polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system are room temperature stable for 4 weeks and give superior PCR performance on complex templates such has mammalian genomic DNA. PCRBIO HiFi Polymerase performs consistently well on a broad range of templates including both GC and AT rich.


Features
  • 50x higher fidelity than Taq DNA polymerase
  • Increased PCR success rates with amplicons up to 10kb
  • Advanced buffer chemistry including Mg and dNTPs
  • High yields under standard and fast PCR conditions
  • Efficient and specific amplification from complex templates including GC rich and AT rich sequences
  • Stable at 25°C and 37°C for 4 weeks

Applications
  • High fidelity PCR
  • Fast PCR – 35 cycles of 5kb amplicon in under 1.5 hours
  • Blunt-end cloning
  • Site directed mutagenesis

Further Applications
  • Long range PCR - up to 10kb
  • Next generation re-sequencing
  • “Difficult” PCR - GC/AT rich DNA
  • Crude sample PCR
  • Colony PCR
WELCOME TO TECHTUM LAB
Since 1975 we have provided laboratories working with DNA, RNA and proteins with innovative instruments and consumables. Techtum is especially appreciated for skilled fast personal contact and a trusted support also after purchase.
 
OUR MOTIVATION AND OBJECTIVES
Our goal is that You, as our customer, is offered innovative/smart/cost effective products to help you in your research or diagnostics.
Our motivation is that You should feel that we are giving you that little extra in the form of better service and support than what you can get elsewhere.
OUR OFFICE 
Techtum Lab AB
Västra Finnbodavägen 4B
SE-131 30 Nacka 
Sweden
 
Phone: +46 (0)90 77 88 80
Email: info@techtum.se
OPENING HOURS
Mon-Thurs: 8:00 to 4:45 p.m.
Fri: 8:00 to 4 p.m.
 
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