PCRBIO HS Taq DNA Polymerase

PCR Biosystems
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• Hot start • Fast and standard cycling • Ultra sensitive


PCRBIO HS Taq DNA Polymerase uses advanced hot start technology for superior sensitivity. Whether you need a hot start assay for high throughput, an automated reaction set up or for detection of a low copy number template, PCR Biosystems offers you a robust industry leading enzyme to meet your needs.

Hot Start
“Hot start” is a term used to describe the inactivation of a DNA polymerase until the initial activation step at 95°C. Inactivation below 65°C prevents primer dimer formation and non-specific amplification allowing for specific amplification from low copy number target sequences. Our proprietary small molecule hot start technology offers improved specificity and sensitivity compared to other methods.

PCRBIO HS Taq DNA Polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system are room temperature stable for 4 weeks and give superior PCR performance on complex templates such has mammalian genomic DNA. PCRBIO HS Taq DNA Polymerase performs consistently well on a broad range of templates (including both GC and AT rich). PCRBIO HS Taq DNA Polymerase production uses an enhanced 12 step purification strategy which includes physical, chemical and enzymatic removal of host DNA.
For added convenience PCRBIO HS Taq DNA Polymerase is also available as a 2x ready mix. PCRBIO HS Taq Mix Red contains a red dye suitable for direct loading and tracking during agarose gel electrophoresis.

Features
  • Proprietary hot start technology for unrivalled detection of low copy number templates
  • Increased PCR success rates with amplicons up to 6kb
  • Ultra low background DNA
  • Advanced buffer chemistry including Mg and dNTPs
  • High yields under standard and fast PCR conditions
  • Efficient specific amplification from complex templates including GC rich and AT rich sequences
  • Inhibitor tolerant PCR direct from bacterial culture, blood and urine
  • Stable at 25°C and 37°C for 4 weeks

Applications
  • Genotyping
  • High throughput PCR
  • Low copy template detection
  • Standard and fast PCR
  • Routine and multiplex PCR
  • TA cloning
  • PCR direct from blood
  • Colony PCR
  • PCR of methylated DNA for bi-sulphite sequencing
  • “Difficult” PCR - GC/AT rich DNA

Available formats
• 5u/μl polymerase + 5x reaction buffer
• 2x ready mix
• 2x ready mix containing red dye for direct gel loading
WELCOME TO TECHTUM LAB
Since 1975 we have provided laboratories working with DNA, RNA and proteins with innovative instruments and consumables. Techtum is especially appreciated for skilled fast personal contact and a trusted support also after purchase.
 
OUR MOTIVATION AND OBJECTIVES
Our goal is that You, as our customer, is offered innovative/smart/cost effective products to help you in your research or diagnostics.
Our motivation is that You should feel that we are giving you that little extra in the form of better service and support than what you can get elsewhere.
OUR OFFICE 
Techtum Lab AB
Västra Finnbodavägen 4B
SE-131 30 Nacka 
Sweden
 
Phone: +46 (0)90 77 88 80
Email: info@techtum.se
OPENING HOURS
Mon-Thurs: 8:00 to 4:45 p.m.
Fri: 8:00 to 4 p.m.
 
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