qPCRBIO HRM Mix

PCR Biosystems
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SNP genotyping • Gene scanning • Ultra sensitive


High Resolution Melt (HRM) analysis is a powerful technique for the analysis of mutations, polymorphisms and epigenetic differences in double stranded DNA (dsDNA) samples. HRM is a post PCR analysis whereby the temperature is increased in small increments, in the presence of a 3rd generation, saturating dsDNA binding dye. As the DNA dissociates (or melts) the dye is released and fluorescence reduces. Fluorescence is measured at regular temperature points to create a melt profile. This profile is sufficiently sensitive to discriminate class I to IV mutations as well as CpG methylation differences.

qPCRBIO HRM Mix
qPCRBIO HRM Mix uses the novel SyGreen 2 dye, a 3rd generation, saturating dsDNA binding dye. Unlike other dsDNA binding
dyes, saturating dyes can be used at saturating concentrations without inhibiting PCR.

Proprietary small molecular inhibitor technology prevents formation of primer dimer and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced
buffer chemistry we offer market leading performance with minimal or no optimisation. High throughput screening has resulted in a buffer system that allows efficient amplification from GC rich and AT rich templates, under fast and standard cycling conditions.

Features
  • 3rd generation saturating dye SyGreen 2
  • Novel hot start for improved sensitivity
  • Compatible on all real-time PCR platforms
  • Standard and fast cycling conditions

Applications
  • Accurate SNP genotyping
  • Gene scanning
  • CpG methylation analysis
  • Efficient specific amplification from GC rich and AT rich sequences
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Since 1975 we have provided laboratories working with DNA, RNA and proteins with innovative instruments and consumables. Techtum is especially appreciated for skilled fast personal contact and a trusted support also after purchase.
 
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