Thermostable reverse transcription • Sensitive • Early Ct
qPCRBIO Probe 1-Step Go is a universal probe kit designed for fast, highly specific and ultra-sensitive probe-based real-time RT-PCR. We use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube.
Fast and Sensitive
qPCRBIO Probe 1-Step Go is engineered for use on a wide range of probe technologies including TaqMan®, Scorpions® and molecular beacons probes. The kit can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences. qPCRBIO Probe 1-Step Go is designed to give rapid and accurate results over a broad range of template concentrations and is ideally suited to the detection of extremely low copy number targets.
Thermostable
The kit includes a thermostable and extremely active modified MMLV reverse transcriptase (RTase Go) and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase. Antibody-mediated hot start technology prevents the formation of primer dimers and non-specific products giving highly specific and ultra-sensitive real-time RT-PCR with unrivalled efficiency in multiplex. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer market-leading performance with minimal or no optimisation.
Features
- High efficiency in multiplex reactions
- Thermostable reverse transcriptase 45°C to 55°C
- Advanced RNase inhibitor
- Rapid extension rate for early Ct values
- Market-leading sensitivity - increased limit of detection
- Antibody-mediated hot start PCR
- Compatible on all real-time PCR platforms - standard and fast cycling conditions
Applications
- Absolute quantification
- Relative gene expression analysis
- TaqMan®, Scorpions® and molecular beacon probes
- Detection of extremely low copy number targets
- Multiplex or singleplex
- Diagnostic real-time PCR
| Catalogue number |
Product name |
Pack size |
Volume |
| 22-PB25.41-01 |
qPCRBIO Probe 1-Step Go Lo-ROX |
100 x 20μl rxns |
[1 x 1ml mix] & [1 x 100μl RTase Go] |
| 22-PB25.41-03 |
|
300 x 20μl rxns |
[3 x 1ml mix] & [3 x 100μl RTase Go] |
| 22-PB25.41-12 |
|
1200 x 20μl rxns |
[12 x 1ml mix] & [12 x 100μl RTase Go] |
| 22-PB25.42-01 |
qPCRBIO Probe 1-Step Go Hi-ROX |
100 x 20μl rxns |
[1 x 1ml mix] & [1 x 100μl RTase Go] |
| 22-PB25.42-03 |
|
300 x 20μl rxns |
[3 x 1ml mix] & [3 x 100μl RTase Go] |
| 22-PB25.42-12 |
|
1200 x 20μl rxns |
[12 x 1ml mix] & [12 x 100μl RTase Go] |
| 22-PB25.43-01 |
qPCRBIO Probe 1-Step Go No-ROX |
100 x 20μl rxns |
[1 x 1ml mix] & [1 x 100μl RTase Go] |
| 22-PB25.43-03 |
|
300 x 20μl rxns |
[3 x 1ml mix] & [3 x 100μl RTase Go] |
| 22-PB25.43-12 |
|
1200 x 20μl rxns |
[12 x 1ml mix] & [12 x 100μl RTase Go] |