Thermostable reverse transcription • Sensitive • Early Ct
qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive real-time RT-PCR. We use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube.
Fast and Sensitive
qPCRBIO SyGreen 1-Step Kits can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences.
Thermostable
The kits include a thermostable and extremely active modified MMLV reverse transcriptase and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase. PCR amplification is powered by antibody-mediated hot start technology, preventing the formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer marketleading performance with minimal or no optimisation.
Features
• Thermostable reverse transcriptase 45°C to 55°C
• Advanced RNase inhibitor
• Non-PCR inhibiting intercalating dye
• Rapid extension rate for early Ct values
• Market-leading sensitivity - increased limit of detection
• Antibody-mediated hot start PCR
• Compatible on all real-time PCR platforms - standard and fast cycling conditions
Applications
• Absolute quantification
• Relative gene expression analysis
• Detection of extremely low copy number targets
qPCRBIO SyGreen 1-Step Detect
qPCRBIO SyGreen 1-Step Detect is designed for sensitivity and is ideally suited to the detection of extremely low copy number
targets. This kit is recommended for template amounts of 1pg - 10ng total RNA or >0.01pg mRNA per reaction.
qPCRBIO SyGreen 1-Step Go
qPCRBIO SyGreen 1-Step Go gives the earliest Ct and is formulated for rapid and accurate results from high templateconcentrations. This kit is recommended for template amounts of 10pg - 100ng total RNA or >0.01pg mRNA per reaction.
qPCRBIO SyGreen 1-Step Detect
qPCRBIO SyGreen 1-Step Detect is designed for sensitivity and is ideally suited to the detection of extremely low copy number
targets. This kit is recommended for template amounts of 1pg - 10ng total RNA or >0.01pg mRNA per reaction.
qPCRBIO SyGreen 1-Step Go
qPCRBIO SyGreen 1-Step Go gives the earliest Ct and is formulated for rapid and accurate results from high templateconcentrations. This kit is recommended for template amounts of 10pg - 100ng total RNA or >0.01pg mRNA per reaction.
| Catalogue number |
Product name |
Pack size |
Volume |
| 22-PB25.11-01 |
qPCRBIO SyGreen 1-Step Detect Lo-ROX |
100 x 20μl rxns |
[1 x 1ml mix] & [1 x 200μl RTase] |
| 22-PB25.11-03 |
|
300 x 20μl rxns |
[3 x 1ml mix] & [3 x 200μl RTase] |
| 22-PB25.11-12 |
|
1200 x 20μl rxns |
[12 x 1ml mix] & [12 x 200μl RTase] |
| 22-PB25.12-01 |
qPCRBIO SyGreen 1-Step Detect Hi-ROX |
100 x 20μl rxns |
[1 x 1ml mix] & [1 x 200μl RTase] |
| 22-PB25.12-03 |
|
300 x 20μl rxns |
[3 x 1ml mix] & [3 x 200μl RTase] |
| 22-PB25.12-12 |
|
1200 x 20μl rxns |
[12 x 1ml mix] & [12 x 200μl RTase] |
| 22-PB25.31-01 |
qPCRBIO SyGreen 1-Step Go Lo-ROX |
100 x 20μl rxns |
[1 x 1ml mix] & [1 x 100μl RTase Go] |
| 22-PB25.31-03 |
|
300 x 20μl rxns |
[3 x 1ml mix] & [3 x 100μl RTase Go] |
| 22-PB25.31-12 |
|
1200 x 20μl rxns |
[12 x 1ml mix] & [12 x 100μl RTase Go] |
| 22-PB25.32-01 |
qPCRBIO SyGreen 1-Step Go Hi-ROX |
100 x 20μl rxns |
[1 x 1ml mix] & [1 x 100μl RTase Go] |
| 22-PB25.32-03 |
|
300 x 20μl rxns |
[3 x 1ml mix] & [3 x 100μl RTase Go] |
| 22-PB25.32-12 |
|
1200 x 20μl rxns |
[12 x 1ml mix] & [12 x 100μl RTase Go] |