qPCRBIO SyGreen 1-Step

PCR Biosystems
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Thermostable reverse transcription • Sensitive • Early Ct

qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive real-time RT-PCR. We use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube.

Fast and Sensitive
qPCRBIO SyGreen 1-Step Kits can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences.

The kits include a thermostable and extremely active modified MMLV reverse transcriptase and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase. PCR amplification is powered by antibody-mediated hot start technology, preventing the formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer marketleading performance with minimal or no optimisation.

• Thermostable reverse transcriptase 45°C to 55°C
• Advanced RNase inhibitor
• Non-PCR inhibiting intercalating dye
• Rapid extension rate for early Ct values
• Market-leading sensitivity - increased limit of detection
• Antibody-mediated hot start PCR
• Compatible on all real-time PCR platforms - standard and fast cycling conditions

• Absolute quantification
• Relative gene expression analysis
• Detection of extremely low copy number targets

qPCRBIO SyGreen 1-Step Detect
qPCRBIO SyGreen 1-Step Detect is designed for sensitivity and is ideally suited to the detection of extremely low copy number
targets. This kit 
is recommended for template amounts of 1pg - 10ng total RNA or >0.01pg mRNA per reaction.

qPCRBIO SyGreen 1-Step Go
qPCRBIO SyGreen 1-Step Go gives the earliest Ct and is formulated for rapid and accurate results from high templateconcentrations. This kit is recommended for template amounts of 10pg - 100ng total RNA or >0.01pg mRNA per reaction.
Since 1975 we have provided laboratories working with DNA, RNA and proteins with innovative instruments and consumables. Techtum is especially appreciated for skilled fast personal contact and a trusted support also after purchase.
Our goal is that You, as our customer, is offered innovative/smart/cost effective products to help you in your research or diagnostics.
Our motivation is that You should feel that we are giving you that little extra in the form of better service and support than what you can get elsewhere.
Techtum Lab AB
Västra Finnbodavägen 4B
SE-131 30 Nacka 
Phone: +46 (0)90 77 88 80
Email: info@techtum.se
Mon-Thurs: 8:00 to 4:45 p.m.
Fri: 8:00 to 4 p.m.
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