Unbiased cDNA synthesis for real-time PCR • Thermostable reverse transcriptase • 30 minute protocol

The qPCRBIO cDNA Synthesis Kit uses the latest developments in reverse transcriptase technology and buffer chemistry to enhance cDNA synthesis speed, yield and representation. The reverse transcriptase, buffer system and combination of random hexamers with anchored oligo(dT) allow for unbiased, efficient and sensitive cDNA synthesis.

High quality cDNA synthesis for downstream qPCR analysis is essential for successful expression studies. Many factors affect cDNA synthesis including the reverse transcripase, buffer systems, enhancers and priming strategy. The PCRBIO cDNA synthesis mix removes the need for user optimisation of these critical factors.

The modified MMLV reverse transcriptase (RTase) is both thermostable and extremely active. The enzyme is blended with RNase inhibitor preventing degradation of RNA by contaminating RNase. The RTase is not inhibited by ribosomal and transfer RNAs, total RNA is an ideal substrate. The 5x cDNA synthesis mix can be used with up to 4μg total RNA.

The relative concentrations of random hexamers and anchored oligo(dT) have been optimised for the generation of cDNA for use in real-time PCR experiments.


Features

• Unbiased representation of 5’ and 3’ mRNA transcript ends
• Sensitive detection of low copy number transcripts
• High cDNA yields from as little as 4pg total RNA
• Simple 2 tube system
• 5x buffer contains anchored oligo(dT), random hexamers, enhancers, dNTPs and MgCl2
• 20x thermostable reverse transcriptase blended with RNase inhibitor

Applications

• cDNA synthesis for real-time PCR analysis
• Low copy number transcripts
• Viral RNA targets
• miRNA targets
• Efficient synthesis from total RNA or poly(A)+ RNA

Catalogue number	Product name	Pack size	Volume
22-PB30.11-02	qPCRBIO cDNA Synthesis Kit	25 Reactions	[1 x 0.1ml] & [1 x 0.025ml]
22-PB30.11-10		100 Reactions	[4 x 0.1ml] & [1 x 0.1ml]