UltraScript Reverse Transcriptase uses the latest developments in reverse transcriptase technology and buffer chemistry to enhance cDNA synthesis speed and yield with accurate transcript representation. The reverse transcriptase buffer system allows for efficient, non-biased and sensitive cDNA synthesis.
UltraScript Reverse Transcriptase is a modified MMLV reverse transcriptase (RTase) that is both thermostable and extremely active. The RTase is not inhibited by ribosomal and transfer RNAs, making total RNA an ideal substrate. The enzyme is blended with RNase inhibitor preventing degradation of RNA by contaminating RNase.
The 5x buffer contains enhancers, dNTPs and MgCl2. It does not contain oligos. The kit can be used with 4.0pg to 0.1μg total RNA or 0.2pg to 2.0μg of oligo(dT) purified mRNA. However, the optimal tempate concentration will ultimately be determined by what oligos are used.
| Component |
10 000 units |
40 000 units |
| 5x UltraScript Buffer |
1 x 200μl |
4 x 200μl |
| UltraScript (200units/μl) (with RNAse inhibitor) |
2 x 25μl |
2 x 100μl |