• Hot start • Fast and standard cycling • Ultra sensitive

PCRBIO HS Taq DNA Polymerase uses advanced hot start technology for superior sensitivity. Whether you need a hot start assay for high throughput, an automated reaction set up or for detection of a low copy number template, PCR Biosystems offers you a robust industry leading enzyme to meet your needs.

Hot Start
“Hot start” is a term used to describe the inactivation of a DNA polymerase until the initial activation step at 95°C. Inactivation below 65°C prevents primer dimer formation and non-specific amplification allowing for specific amplification from low copy number target sequences. Our proprietary small molecule hot start technology offers improved specificity and sensitivity compared to other methods.

PCRBIO HS Taq DNA Polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system are room temperature stable for 4 weeks and give superior PCR performance on complex templates such has mammalian genomic DNA. PCRBIO HS Taq DNA Polymerase performs consistently well on a broad range of templates (including both GC and AT rich). PCRBIO HS Taq DNA Polymerase production uses an enhanced 12 step purification strategy which includes physical, chemical and enzymatic removal of host DNA.
For added convenience PCRBIO HS Taq DNA Polymerase is also available as a 2x ready mix. PCRBIO HS Taq Mix Red contains a red dye suitable for direct loading and tracking during agarose gel electrophoresis.

Features

• Proprietary hot start technology for unrivalled detection of low copy number templates
• Increased PCR success rates with amplicons up to 6kb
• Ultra low background DNA
• Advanced buffer chemistry including Mg and dNTPs
• High yields under standard and fast PCR conditions
• Efficient specific amplification from complex templates including GC rich and AT rich sequences
• Inhibitor tolerant PCR direct from bacterial culture, blood and urine
• Stable at 25°C and 37°C for 4 weeks

Applications

• Genotyping
• High throughput PCR
• Low copy template detection
• Standard and fast PCR
• Routine and multiplex PCR
• TA cloning
• PCR direct from blood
• Colony PCR
• PCR of methylated DNA for bi-sulphite sequencing
• “Difficult” PCR - GC/AT rich DNA

Available formats
• 5u/μl polymerase + 5x reaction buffer
• 2x ready mix
• 2x ready mix containing red dye for direct gel loading

Catalogue number	Product name	Pack size	Volume
22-PB10.21-02	PCRBIO HS Taq DNA Polymerase	250 Units	[1 x 0.05ml 5 units/μl] & [2 x 1ml buffer]
22-PB10.21-10		1000 Units	[4 x 0.05ml 5 units/μl] & [8 x 1ml buffer]
22-PB10.21-50		5000 Units	[20 x 0.05ml 5 units/μl] & [40 x 1ml buffer]
22-PB10.22-02	PCRBIO HS Taq Mix	200 Reactions	5 x 1ml
22-PB10.22-10		1000 Reactions	5 x (5 x 1ml)
22-PB10.23-02	PCRBIO HS Taq Mix Red	200 Reactions	5 x 1ml
22-PB10.23-10		1000 Reactions	5 x 1ml