Sensitive - Fast - Specific
PCR Biosystems use a proprietary intercalating dye that does not inhibit PCR, unlike other popular fluorescent dyes. Combined with advanced enzyme, hot start and reaction buffer technology we offer market-leading sensitivity and reproducibility.
qPCRBIO SyGreen Mix can be used to quantify any DNA template including genomic, cDNA and viral sequences. Extremely low copy number targets can be detected specifically and with high efficiency. Antibody-mediated hot start technology prevents the formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer marketleading performance with minimal or no optimisation.
Features
- Non-PCR inhibiting intercalating dye, better signal
- Rapid extension rate for early Ct values
- Market leading sensitivity - increased limit of detection
- Compatible on all real-time PCR platforms - standard and fast cycling conditions
- Blue mix available for easy sample visualisation during pipetting
Applications
- Absolute quantification
- Relative gene expression analysis
- High throughput qPCR from genomic, cDNA and viral sequences
- Low copy number target genes
Further Applications
- Crude sample PCR
- Standard and fast PCR conditions
- Specific amplification from complex templates (eg GC/AT rich)
- Compatible with all real-time PCR instruments
| Catalogue number |
Product name |
Pack size |
Volume |
| 22-PB20.11-01 |
qPCRBIO SyGreen Mix Lo-ROX |
100 x 20μl rxns |
1 x 1ml |
| 22-PB20.11-05 |
|
500 x 20μl rxns |
5 x 1ml |
| 22-PB20.11-20 |
|
2000 x 20μl rxns |
20 x 1ml |
| 22-PB20.12-01 |
qPCRBIO SyGreen Mix Hi-ROX |
100 x 20μl rxns |
1 x 1ml |
| 22-PB20.12-05 |
|
500 x 20μl rxns |
5 x 1ml |
| 22-PB20.12-20 |
|
2000 x 20μl rxns |
20 x 1ml |
| 22-PB20.13-01 |
qPCRBIO SyGreen Mix Fluorescein |
100 x 20μl rxns |
1 x 1ml |
| 22-PB20.13-05 |
|
500 x 20μl rxns |
5 x 1ml |
| 22-PB20.13-20 |
|
2000 x 20μl rxns |
20 x 1ml |
| 22-PB20.14-01 |
qPCRBIO SyGreen Mix Separate-ROX |
100 x 20μl rxns |
[1 x 1ml mix] & [1 x 150μl ROX] |
| 22-PB20.14-05 |
|
500 x 20μl rxns |
[5 x 1ml mix] & [1 x 150μl ROX] |
| 22-PB20.14-20 |
|
2000 x 20μl rxns |
[20 x 1ml mix] & [4 x 150μl ROX] |